Figure 4
Western blot analysis of p53 accumulation and PARP cleavage in HeLa and MON cells treated with 40 μM CisPt for 2 h or irradiated with 20 J/m2 UVC and incubated for different time intervals before the sample processing. These times (hours) were noted above the lanes. C refers to untreated controls. Lysates from 5.105 cells were subjected to SDS-PAGE (8%), blotted and incubated with a monoclonal antibody to p53 or PARP. The data are representative of two (PARP) to five (p53) independent experiments.