Epithelial Na, K-ATPase expression is down-regulated in canine prostate cancer; a possible consequence of metabolic transformation in the process of prostate malignancy

Table 1 Isoform specific antibodies used to detect the isoforms of Na, K-ATPase in canine prostatic tissue by immunohistochemical analysis.

Antibody	Isoform Specificity	Working Dilution	Source	References
α5^§	Recognizes all	Neat Supernatant	D. M. Fambrough	[32]
Pan α (monoclonal)	known α isoforms
mAb9A7	Recognizes all	1:100	M. Takahashi	[21]
Pan α (monoclonal)	known α isoforms
α620 (polyclonal)	α1 specific	1:200	M.J. Caplan	[33]
McB2 (monoclonal)	α2 specific	Neat Supernatant	K.J. Sweadner	[34]
XVIF9G10 (monoclonal)	α3 specific	Neat Supernatant	K.P. Campbell	[35]
SpETb1 (polyclonal)	β1 specific	1:200	P. Martín-Vasallo	[36]
SpETb2 (polyclonal)	β2 specific	1:200	P. Martín-Vasallo	[36]
γC33 (polyclonal)	γ specific	1:200	S.J.D. Karlish	[19]

^§ The α5 monoclonal broadly recognizes Na⁺, K⁺-ATPase α subunits of avian, mammalian and insect species. There are 4 known α isoforms and the designation " α5" does not indicate the existence of a fifth Na⁺, K⁺-ATPase α isoform. The α5 antibody developed by D. Fambrough was also obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biological Sciences, Iowa City, IA 52242, U.S.A. The monoclonal antibody XVIF9G10 developed by K.P. Campbell http://www.physiology.uiowa.edu/campbell/public/Antibodies.htm is commercially available from Affinity Bioreagents.

ISSN: 1475-2867