Figure 3

Inhibition of phospholipase C activity causes release of Hsp70 from A431 cells. The left panel: conditioned medium from A431 cells, control and treated with U73122 PLC inhibitor, was passed over the ATP-agarose column and the eluted protein was subjected to immunoblotting, using 2H9 antibody. The central and right panels: samples of conditioned medium were incubated with 2H9 antibody attached to Protein G-Sepharose, and the immunoprecipitated protein was studied with the help of immunoblotting, using 2H9 antibody (central panel) or anti-ubiquitin antibody (right panel). The lower panel: immunoprecipitation/immunoblotting with lysates of control and U73122-treated cells.