Figure 3
From: Functional and structural characteristics of anticancer peptide Pep27 analogues
Determination of apoptosis and membrane integrity by FACS. Cells were treated with etoposide, mellitin and Pep27 anal2. Apoptosis and membrane integrity were determined using annexin V and propidium iodide (PI), respectively, as described in Materials and Methods. A, control; B, 100 μM etoposide; C, 12.5 μM mellitin; D, 12.5 μM Pep27anal2; E, 62.5 μM Pep27anal2. Left, dual staining for annexin V-FITC (FL1-H) and PI (FL2-H); Middle, distribution of annexin V-FITC staining in cell populations; Right, distribution of PI staining. Similar results were obtained in independent duplicated experiments.