Figure 8
Effect of over-expression of ZIP1-Myc on RWPE2 cell cycle progression and apoptosis. a. Effect of over-expression of ZIP1 on cell cycle. Vector or ZIP1-Myc expressing RWPE2 cells were plated and grown for 24 hours. Cells were harvested and counted. 2 × 106 cells were ethanol fixed and processed for DNA staining with propidium Iodine. Cells were run on a FACS SCAN (BD Dickenson) with a 488 nm argon laser light source. 10,000 events were counted per sample. Values are the means ± SD of one representative experiment of two with similar results. b. Effect of over-expression of ZIP1 on apoptosis. 2 × 106 vector or ZIP1-Myc expressing RWPE2 cells were plated in 100 mm plates and grown for 24 hours. Cells were treated with TRAIL (10 ng/ml) at 37°C for 6 hours. Cells were harvested by trypsinization. Caspase 3 activity was determined according to the manufacture's instructions (Clontech ApoAlert Caspase Colorimetric Assay Kit). Values are means ± SD of one representative experiment of two with similar results.