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Figure 1 | Cancer Cell International

Figure 1

From: "Nutritional and chemopreventive anti-cancer agents up-regulate expression of p27Kip1, a cyclin-dependent kinase inhibitor, in mouse JB6 epidermal and human MCF7, MDA-MB-321 and AU565 breast cancer cells"

Figure 1

Nutritional and chemopreventive anti-cancer agents up-regulate the activity of the proximal 5-upstream region (-1797) of p27 gene in a manner specific to p27. (a) Preliminary studies suggesting – but not proving – that various nutritional and chemopreventive anti-cancer agents, including moderate caloric/dietary restriction, up-regulate p27 expression. (b) Schematic drawing of the proximal 5'-upstream region of the genes used in this experiment: (c) -1745 cyclin D, (d) -8100 cyclin A, (e) -1797 p27 (p27-Kpn I), and (f) -2320 p21. The JB6 cells, transfected with these proximal 5'-upstream region of the genes, were exposed to all-trans-retinoic acid (atRA) (1 μM), 9-cis-retinoic acid (9cRA) (1 μM), 13-cis-retinoic acid (13cRA) (1 μM), phorbol 12-myristate 13-acetate (TPA) (16.2 nM), 1α, 25-dihydroxyvitamin D3 (calcitriol) (240 nM), or dexamethasone (1 μM) for 24 hours. All assays were performed in triplicates and the transfection of JB6 cells with each 5'-upstream region of the genes was repeated three times.

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Cancer Cell International

ISSN: 1475-2867

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