From: Ultraviolet-A radiation induces changes in cyclin G gene expression in mouse melanoma B16-F1 cells
Gene family [Swiss prot Accession #] | No. of arraysa | Controlb± SD | UVA exposedc± SD | t-test | Ratiod | Function |
---|---|---|---|---|---|---|
Stress induced | ||||||
Heat shock 86-kDa protein (HSP86) [P07901] | 3 ↑ | 0,32 ± 0,23 | 1,04 ± 0,44 | 0,43 | 3,23 | Belongs to HSP90 family; cytoplasmic molecular chaperone regulating the correct folding in the heat induced conformational changes. |
Heat shock cognate 71-kDa (HSC70; HSP73), mouse homolog of human [P11142] | 2 ↑ 1 ↓ | 0,15 ± 0,12 | 0,27 ± 0,18 | 0,39 | 1,81 | Belongs to HSP70 family; molecular chaperone regulating the correct folding; found in melanoma cell lines [33]. |
Alpha crystallin B-subunit, mouse homolog of human [P02511] | 3 ↑ | 0,07 ± 0,03 | 0,21± 0,07 | 0,05 | 3,26 | Belongs to HSP20 family; found in mammalian transparent lens in eye induced by stress [34,35]. |
Oxidative Stress | ||||||
Oxidative stress-induced protein (OSI) [Q64337] | 2 ↑ 1 ↓ | 0,16 ± 0,14 | 0,31± 0,15 | 0,27 | 1,92 | Regulates metabolic responses to oxidative stress, were also induced [36] |
glutathione S-transferase mu2 (GSTM2); [P15626] | 3 ↑ 1 ↓ | 0,07 ± 0,04 | 0,14 ± 0,09 | 0,23 | 2,09 | Belongs to the GST superfamily; prevents the toxic injuries; expressed in human melanoma cells [37] and in keratinocytes from human squamous cell carcinoma [38]. |
Cell cycle control | ||||||
cyclin G [O54779; P51945] | 5 ↑ | 0,13 ± 0,04 | 0,65 ± 0,36 | 0,03 | 4,85 | Contributes to G2/M arrest in response to DNA damage; a transcriptional target of the p53. |
Angiogenesis | ||||||
Vascular endothelial growth factor (VEGF) [Q00731] | 4 ↑ | 0,07 ± 0,03 | 0,22 ± 0,08 | 0,03 | 2,94 | Promotes endothelial cell proliferation and migration in angiogenesis; permeabilizes the blood vessels; expression regulated by UVB in keratinocytes and fibroblasts [39,40]. |
Skeleton & Motility protein | ||||||
G-actin cytoplasmic [P02571; P14104] | 3 ↓ | 1,02 ± 0,37 | 0,47 ± 0,18 | 0,10 | 0,47 | Exists in all eukaryotic cells as a component of the cytoskeleton mediating cell motility. Polymerization leads to formation of filamentous F-actin. |
non-muscle cofilin 1 (CFL1) [P18760] | 3 ↓ | 0,40 ± 0,10 | 0,17 ± 0,01 | 0,05 | 0,41 | Controls reversibly actin depolymerization from F-actin to G-actin causing an increase in the G-actin pool. |