Figure 3

Suppression of H3-K9 or H3-K27MT attenuated anchorage independent cell growth ability of transformed NHBE cells. A. Anchorage independent growth on soft agar is inhibited in Ras transformed human bronchoepithelial cells knocked down with all H3K9 MTs, SETDB1, G9A and SUV39H or H3K27 MT, EZH2 compared with the cells transfected with siRandom non-targeting control, indicating that SETDB1 is only required for anchorage independent growth as it did not affect general cell growth while other H3K9 MTs are required for cell growth. Suppression of transcription activating H3KMTs; SET9, SMYD3 or DOT1L did not reduce colony formation in soft agar. The experiments are done in triplicates and repeated twice. Columns, averages; bars, SEM. B. Photographs of representative wells.