Fig. 5

The cytometric analysis of cell cycle distribution using propidium iodide staining. The percentage of cells a with <4N DNA content, b in G0/G1 phase, c in S phase, d in G2/M phase, and e with >4N DNA content. The A549 cells were treated with 10 µM fisetin (FIS) and/or 0.1 µM paclitaxel (PTX) for 6, 10 or 30 h. In another set of experiment, the cells were treated with the combination of 10 µM fisetin and 0.1 µM paclitaxel for 30 h, followed by post-treatment incubation in a drug-free medium for the next 24 or 48 h. Control cells (CTRL) were cultured under identical conditions, but without the addition of the tested agents. Asterisks represent statistically significant differences from control cells (p < 0.05; Mann–Whitney U test). Symbols ^$ and ^# indicate statistically significant differences compared with FIS or PTX treatment alone, respectively (p < 0.05; Mann–Whitney U test). The data are representative of five independent experiments and presented as mean and standard deviation