Fig. 5

Changes in expression of differentiation-related genes in osteosarcoma cell lines after treatment with MTX. The relative expression of selected genes in Saos-2 (a), OSA-02 (b), OSA-03 (c), OSA-05 (d), OSA-06 (e) and OSA-08 (f) cells measured using RT-qPCR at day 3 of incubation with 1 μM MTX (1MTX) or with 40 μM MTX (40MTX). The levels of relative gene expression are presented as fold changes compared to the levels detected in control samples. The data represent the mean ± SD. Experiments were repeated three times. *P < 0.05, **P < 0.01, indicate significant differences from the respective control groups