Fig. 2
From: Suppression of Spry4 enhances cancer stem cell properties of human MDA-MB-231 breast carcinoma cells
Suppressing Spry4 expression promotes MDA-MB-231 tumor growth and lung metastasis. a Representative images of soft-agar colony formation assays show that S4kd cells formed more colonies compared to NT cells. b Quantification of soft-agar colony formation assay. c Representative images of tumors harvested at 9 weeks after fat pad inoculation of 1 × 106 NT or S4kd#1 cells. d Tumor growth curve was present with average tumor volume from five animals in each group. e Representative H&E staining of lungs from 1 × 106 dosage xenograft mice showing more and larger metastasis lesions in S4kd injected mice compared to NT injected mice. f Quantification of lung metastasis. g RT-qPCR analysis of human HRPT transcript versus total 18S rRNA transcripts in lungs from S4kd and NT cells injected mice, the relative mRNA level of human HRPT in S4kd tumors compare to NT tumors is presented. *p < 0.05, **p < 0.01