Skip to main content
Fig. 1 | Cancer Cell International

Fig. 1

From: In vivo enhancement of the MAGE-specific cellular immune response by a recombinant MAGE1-MAGE3-TBHSP70 tumor vaccine

Fig. 1

Vaccine information and MAGEs expression in tumor cell lines. A The expression vector for the recombinant vaccine protein including MAEG1-TBHSP70 expression vector (abbr. pET-M1H), MAGE3-TBHSP70 vector (abbr. pET-M3H) and MAGE1-MAEG3-TBHSP70 (abbr. pET-MMH) expression vector. B Protein lysates were run through electrophoresis gel and stained with Coomassie. C Purified recombinant proteins were run through electrophoresis gel and transferred into PVDF membrane, and then blotted with MAGE1 and MAGE3, showing similar pattern as coomassie staining. D Purified recombinant protein were blot with MAGE1 and MAGE3 separately. E Protein level of MAGE1 and MAGE3 in three different tumor cell line including HepG2, A549 and HHCC. F mRNA transcription levels of MAGE1 and MAGE3 in HepG2, A549 and HHCC cell lines using nest-RT-PCR

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us