Up-regulation of CRKL by microRNA-335 methylation is associated with poor prognosis in gastric cancer

Table 2 Association between the promoter region methylation status of miR-335 and clinicopathologic features in patients with gastric cancer

	Hypermethylation (n = 127)		Hypomethylation (n = 104)		P
	n	%	n	%
Age					0.830
>55	98	77	79	76
≤55	29	23	25	24
Sex					0.371
Male	71	56	52	50
Female	56	44	52	50
Histological grade					0.765
Well	44	35	38	37
Poor	83	65	66	63
Tumor size (cm)					0.004*
≤3.5	25	20	38	37
>3.5	102	80	66	63
Macroscopic type					0.843
Early stage	4	3	2	2
Borrmann I + II	13	10	11	11
Borrmann III + IV	110	87	91	87
Lauren's grade					0.347
Intestinal	44	35	30	29
Diffuse	83	65	74	71
Venous invasion					0.922
Absent	97	76	80	77
Present	30	24	24	23
Liver metastasis					0.622
Absent	116	91	93	89
Present	11	9	11	11
Peritoneal dissemination					0.223
Absent	108	85	94	90
Present	19	15	10	10
pT stage					0.434
T1	3	2	3	3
T2	23	18	21	20
T3	35	28	37	36
T4	66	52	43	41
pN stage					0.240
N0 + N1	36	28	37	36
N2 + N3	91	72	67	64
Metastasis lymph node					0.058
Negative	30	24	37	35
Positive	97	76	67	65
Invasion into lymphatic vessels					0.001*
Negative	93	73	94	90
Positive	34	27	10	10
AJCC stage					0.197
I + II	60	47	58	56
III + IV	67	53	46	44
MiR-335 level					<0.001*
Low expression	78	61	37	36
High expression	49	39	67	64

ISSN: 1475-2867