Fig. 3

The growth-suppressive effect of VEGFA silencing and bevacizumab on MMQ tumor stem-like cells in vitro. a, b The effect of VEGFA knockdown via siRNA silencing in TSLC and MMQ cells. The cells were transfected with siControl or siVEGFA for 72 h and subjected to quantitative PCR and immunoblot analysis of VEGFA expression. c VEGFA knockdown via siRNA silencing inhibited the proliferation of TSLC. The cells were transfected with siControl or siVEGFA for 24–96 h and subjected to cell proliferation assay by CCK-8. d 10,000 MMQ TSLC, treated with bevacizumab (4 mg/μl) or DMSO for 72 h, were plated into each well of a 96-well plate and subjected to cell proliferation assay by CCK-8. The viability of MMQ TSLC treated with bevacizumab decreased compared with the negative DMSO group. TSLC: tumor stem-like cell. *P < 0.05, **P < 0.01, ***P < 0.001