Fig. 2

miR-484 suppresses cell cycle of CC cells and exacerbates apoptosis. a, b The cell cycle progression of HeLa cells was analyzed by flow cytometry. The chart shows the population of cells in G1-, S- and G2-phase (a) as well as the proliferation index (b). Proliferation index = (S + G2)/G1 (S, G2 and G1 are the percentages of cells in S-phase, G2-phase and G1-phase, respectively). c After transfection 24 h, apoptosis of HeLa cells was monitored by Annexin V-PI double staining and flow cytometry assay. Paclitaxel was used to induce cell apoptosis 6 h before collecting cells. The right lower quadrant of each plot contains early apoptotic cells, whereas the right upper quadrant contains late apoptotic cells. All data represent mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001