Fig. 5

The ectopic expression of SMAD2 and ZEB1 counteracts the inhibition of cell proliferation induced by miR-484. a CC cells were co-transfected with pcDNA3/pri-miR-484 and pFlag-SMAD2/ZEB1 without its 3′UTR or the control vector and western bolt assay was used to determine the restoration of SMAD2 or ZEB1 protein levels by pFlag-SMAD2/ZEB1 in the presence of miR-484. b After transfection 48 h, MTT assay was performed to detect cell viability in CC cells. c The transfected cells were submitted to colony formation assays to test the proliferation of CC cells. All data represent mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001