Fig. 1

EphB2 is expressed in both medulloblastoma patient samples from different medulloblastoma subtypes and cell lines. a Transcriptomic data analysis show that EphB2 receptor is overexpressed in four distinct medulloblastoma subtypes compared to normal counterparts. Similar trend was evident with the mRNA analysis performed on EPHB2 expression by using the R2 platform (http://r2.amc.nl). The Medulloblastoma Gilbertson (GSE37418) dataset (b) and Kool (GSE10327) dataset (c) were used for the analysis [15]. For normal expression the Normal Cerebellum Roth dataset (GSE3526) was utilized. The subgroups in the Kool’s dataset are labeled based on the published literature [16, 17]. One-way analysis of variance was performed to compare EPHB2 expression across medulloblastoma subgroups with normal cerebellum expression. d IHC analysis show that EphB2 is expressed at high levels in medulloblastoma patient samples compared to the normal cerebellum. e EphB2 expression is decreased following transfection with EphB2-specific siRNA alone and in the presence of 8 Gy dose of radiation compared to control non-specific siRNA (NS-siRNA) at 72 h post-XRT