Fig. 3From: pATM and γH2AX are effective radiation biomarkers in assessing the radiosensitivity of 12C6+ in human tumor cellsFoci formation of γH2AX and pATM in Hela, HepG2 and MEC-1 cells observed by immunofluorescent microscopy. The three cell lines are exposured to 0.5, 1, 2 and 4 Gy 12C6+ and subsequently incubated for 0.5, 4 and 24 h for γH2AX and pATM in vitro. a, b, c γH2AX; d, e, f pATM; a, d Hela cells; b, e HepG2 cells; c, f MEC-1 cells. *P < 0.05 vs. 0 Gy irradiation; **P < 0.01 vs. 0 Gy irradiation. Over 800 randomly selected cells were counted. Cells with three or more foci of any size were classified as positive. Results are the means and SD for the three experimentsBack to article page