Fig. 3

27-OHC upregulates ERβ expression. Representative western blots (a) and densitometric analysis showing no significant change in ERα expression in RWPE-1 (b) and a significant increase in ERβ expression (c) in RWPE-1 cells. Representative western blots (d) and densitometric analysis showing no significant change in ERα expression (e) and a significant increase in ERβ expression in LNCaP cells. Representative western blots (g) and densitometric analysis showing no significant change in ERα expression (h) and a significant increase in ERβ expression in PC3 cells (i). Data is expressed as mean ± SEM. *p < 0.05 versus controls