Fig. 1
The effect of BA on cell viability. a Structure of BA. Chemical formula: C21H17ClN2O; molecular weight: 348.5. b, d SMMC-7721 and LO2 cell viability at 24th, 48th, and 72th hour after BA treatment. SMMC-7721 and L02 cells were cultured in 96-well plates in the presence of a series of BA concentrations: 0, 4, 8, 16, 32 and 64 μM. Cell viability was measured by MTT assay. The data was obtained from three independent experiments and was presented as mean ± SD (*p < 0.05, BA vs. control). c, e Colony formation of SMMC-7721 cells in BA treatment. BA concentrations were 2, 4, and 8 μM. No BA treatment cells were used as negative control. SMMC-7721 cells (1*103 cells/well) were seeded and cultured in the absence or presence of BA at increasing concentrations (0, 2, 4, and 8 μM) for 24 h. Colonies were stained with crystal violet and quantified by densitometry (*p < 0.05, BA vs. control). c Representative pictures of colonies formed. e Percentage of colonies formed per well