Fig. 4

Mechanistic studies of BA anti-hepatocellular carcinoma effect. a BA induced caspase 3 expression but reduced the caspase 9 level in SMMC-7721 cells. SMMC-7721 cells were treated with increasing concentrations of BA (0, 2, 4, 8, 16 μM) for 24 h before analysis; β-actin was used as a loading control. b P-Akt and Akt expression in SMMC-7721 and LO2 cells treated with increasing concentrations of BA (0, 2, 4 μM) for 24 h