Fig. 2

Biological characteristics of meningioma primary cell lines. a Observed morphological characteristics showing cells displaying different phase and Vimentin stained structures. M Type round, mitotic like, (10–60 µm); N Type neuronal-like, bipolar, spindle like, (100–500 µm in length); O Type flat and short spikes or processes, oligodendrocytic-like (10–100 µm); G Type large, cytoplasmic region, with no/few processes, glial-like (100–500 µm); A Type many long spikes or processes with visible central cytoplasm, astrocytic-like (100–700 µm Length of processes); and D Type oval and dark phase centered (20–60 µm). Images were taken at ×20 magnifications. b Average percentages of morphologies counts for all primary cell lines. Horizontal accesses show the different morphologies mentioned in a. Two groups emerged, cell lines that had predominantly G type cells (9 cell lines) or those that had a mix composition of N and G type cells (5 cell lines). c Average doubling time for G type and NG type cell lines at early passages. d Average percentage of Ki67 positive cells in four G and two NG cell lines. e An in vitro scratch assay for two G type and NG type cell lines. Cells on the edge of the newly created gap moved toward the opening to close the “scratch” within a day in NG type cell lines. Images were taken at ×5 magnifications