Fig. 2From: Mechanism study of oleanolic acid derivative, K73-03, inducing cell apoptosis in hepatocellular carcinomaInduction of apoptosis by K73-03 in HepG2 cells. (A) Apoptotic morphological changes on HepG2 after K73-03 treatment (0, 2, 4 and 8 µM) using DAPI staining. (B) TEM observed the occurrence of apoptosis in HepG2 cells after treatment with 8 µM K73-03 for 24 h. (C) The evaluation on apoptotic rate using Annexin V-FITC and PI double staining analyzed by flow cytometry. (D) The expressions of cleaved-caspase-9, cleaved-caspase-3, Bax and Bcl-2 protein were determined by Western blotting. The data were presented as the mean ± SD of three independent experiments. (*p < 0.05, **p < 0.01, significantly different from control group)Back to article page