Fig. 7

Inhibitory effect of TNFRSF21 on osteosarcoma. Subcutaneous tumors were dissected and photographed after 4 weeks, P value was calculated by 2-tailed Student t test (n = 6) (A). EdU staining (green fluorescence) on cell proliferation 2 days after the construction of osteosarcoma cell lines overexpressing TNFRSF21, nuclei were counterstained blue with DAPI (Scale bars, 50 μm) (B). Cell proliferation was detected by measuring the optical density in 450 nm, P value was calculated by 2-tailed Student t test (n = 4) (***, P < 0.001) (C). Representative images of colony formation assays in eighth day after the construction of osteosarcoma cell lines overexpressing TNFRSF21, P value was calculated by 2-tailed Student t test (n = 3) (***, P < 0.001) (D). Representative images of wound healing assays after 12 h of the gap formation (Scale bars, 500 μm) (E). Representative images of Transwell assays (migration and invasion) after 24 h incubation (Scale bars, 100 μm), P value was calculated by 2-tailed Student t test (n = 3) (*, P < 0.05; ***, P < 0.01; ***, P < 0.001) (F)