Fig. 1
Effect of microglia on brain tumor cells. a The diagram of cell co-culture experiment designation indicates high-density (3.2 × 103/mm2), medium-density(1.6 × 103/mm2), and low-density(0.8 × 103/mm2) seeding conditions. b The images of co-culture on different seeding intensities after 24 h of incubation. The blue arrow indicates the colony (a cluster of diameter > 50 μm). Scale bar = 200 μm. c Statistics of the average colony diameter among different conditions. d The immunofluorescence staining of ALTS1C1-GFP (Shown in green) co-culture with BV2 for 24 h. The nucleus was stained with Hoechst (Left picture, shown in Blue), and the cells were stained with myeloid cell marker CD11b (Right, shown in red). Scale bar = 200 μm. e The images of ALTS1C1 were cultured in different condition mediums for 24 h of incubation. Scale bar = 100 μm. f The images of different cancerous cell lines co-cultured with BV2 for 24 h. Scale bar = 200 μm. A two-tailed unpaired t-test was used to compare every two groups. ****P < 0.0001