Fig. 2

Chemo-resistance of ALTS1C1 after co-culturing with BV2. a The MTT cytotoxicity assay of the pro-drug GCV on ALTS1C1, ALTS1C1-Tk, and BV2 cell lines for 72 h of incubation. b Representative dot plot FACs images of the co-culture chemo-apoptosis assay of 10 μg/mL pro-drug GCV on ALTS1C1-Tk only or ALTS1C1-Tk (+BV2) for 0, 24, 36 h. c Quantification of the chemo-apoptosis assay. N ≧ 3. d Representative caspase-3 staining images of ALTS1C1-GFP-Tk, ALTS1C1-GFP-Tk co-cultured with BV2 treated with 10 μg/mL GCV for 24 h. Scale bar = 200 μm. e Quantification data of the caspase-3 staining. N = 3. A two-tailed unpaired t-test was used to compare every two groups. *P < 0.05, **P < 0.01