Fig. 4

BV2 hijacks drugs from ALTS1C1 through direct contact. a The experimental scheme on the Dox transfer test between the ALTS1C1 and BV2 cells. b Representative flow images of PE-positive cell percentages indicating Dox-positive cells (Left), and the quantification of Dox-positive cells after co-culturing for 4 h (Right). c In-direct transfer experiment scheme. d Representative flow intensity images of in-direct transfer experiment (Left), and the quantitative data of median fluorescence intensity (MFI) ratio of trans-well (in-direct) and direct contact groups. N = 3. e Representative flow intensity images of cells treated with Dox for 4, 12, and 24 h. f The quantification of median fluorescence intensity (MFI) of Dox on ALTS1C1, and ALTS1C1 (Co-cultured with BV2) for 4, 12, and 24 h. g Representative images of time-lapse picturing Dox transferring from ALTS1C1-GFP to BV2. Images were taken every 5 min. Scale bar = 25 μm. A two-tailed unpaired t-test was used to compare every two groups. **P < 0.01. ***P < 0.001, ****P < 0.0001