Fig. 5

The effect of gap junction on co-culture induced chemoresistance. a The representative images of various concentration CBX on ALTS1C1-Tk and BV2 co-culture. Scale bar = 500 μm. b The representative flow images of chemo-apoptosis assay on different concentrations of CBX on ALTS1C1-Tk and BV2 co-culture. c statistics of chemo-apoptosis assay, N = 3. d Representative dot plot FACs images of the co-culture chemo-apoptosis assay of 10 μg/mL pro-drug GCV and 10 μM CBX on ALTS1C1-Tk only or BV2 co-cultured group for 0, 24, 36 h. e Quantification of the chemo-apoptosis assay, the protective effect has significantly weakened under CBX treatment. N = 3. A two-tailed unpaired t-test was used to compare every two groups. *P < 0.05