Fig. 2

USP5 overexpression promotes bladder cancer cell proliferation, viability and migration. A EJ cells were transfected with FLAG-USP5 or FLAG-USP5 C335A as indicated, and protein levels were measured by western blotting. GAPDH served as a control. B EJ cells were transfected with USP5 or FLAG-USP5 C335A as indicated, and wild-type EJ cells served as controls. Colony formation assays were performed to evaluate cell viability. The colonies were stained with crystal violet and photographed. The number of colonies was determined and plotted (n = 3). C EJ cells were transfected with FLAG-USP5 or FLAG-USP5 C335A as indicated, and wild-type EJ cells served as controls. CCK-8 assays were used to analyse cell proliferation (n = 6). D EJ cells were transfected with FLAG-USP5 or FLAG-USP5 C335A as indicated, and wild-type EJ cells served as controls. Scratch assays were used to evaluate the effects of USP5 overexpression on cell migration and wound repair. The cells were imaged (left, ×20 magnification) and counted, and the results were plotted (right, n = 3). The data (mean ± SEM) are representative of 3 independent experiments. E EJ cells were transfected with FLAG-USP5 or FLAG-USP5 C335A as indicated, and wild-type EJ cells served as controls. Transwell experiments were used to evaluate the effects of USP5 overexpression on cell migration. The cells were imaged (left, ×20 magnification) and counted, and the results were plotted (right, n = 3). The data (mean ± SEM) are representative of 3 independent experiments. Statistical significance was analysed by ANOVA or Student’s t test. *P < 0.05, **P < 0.01, ****P < 0.0001