Fig. 6

Effects of GSK-J4 on a metastatic mouse model. Tumor metastatic dissemination post intravenous injection of siH19-PC-3 and Vector cells in presence or absence of GSK-J4 as in the legend to Fig. 5. (A) Number of bone metastasis (mets)/mouse. Data represent the mean +/- SEM of 12 mice/group (white dots). Statistical significance was determined by the Chi-square test, 2-tail, on the proportion of the number of mets/mouse. *P < 0.05. (B) Lung metastasis measured as photons/sec in Region Of Interest (ROI) on the day of explant. Data represent mean +/- SEM of 12 mice/group (white dots). (C) Representative H&E-stained sections of the liver. Dashed lines indicated the zoom area in the right panels. Color lines indicate metastasis diameter. Scale bars:2 mm and 500 μm. Right: Tumor growth measured in the liver as diameter (µm) on H&E staining. Data represent mean +/- SEM of 12 mice/group (2 or 3 metastasis/mouse, white dots). *P < 0.05