Fig. 2
From: circ-TFRC downregulation suppresses ovarian cancer progression via miR-615-3p/IGF2 axis regulation
Downregulation of circ-TFRC inhibited OC cell proliferation and tumor growth. (A) RT-qPCR detection shows the expression of circ-TFRC in both SKOV3 and A2780 cells after transfection with NC and circ-TFRC silence vector (sh-circ-TFRC). The data are expressed as the mean ± SD. ***P < 0.001 versus NC. n = 3. (B and C) CCK8 detection showed the proliferation of both SKOV3 and A2780 cells. The data are expressed as the mean ± SD. ***P < 0.001 versus NC. n = 3. (D and E) EdU detection showed the proliferation of both SKOV3 and A2780 cells. The data are expressed as the mean ± SD. ***P < 0.001 versus NC. n = 3. (F) Summary of tumor volumes. The data are expressed as the mean ± SD. ***P < 0.001 versus NC. (G) Representative photographs of SKOV3 tumor formation in xenografts of nude mice. n = 5. (H) Summary of tumor weight in mice. The data are expressed as the mean ± SD. ***P < 0.001 versus sh-NC. (I and J) IH showed the percentage of Ki-67-positive cells. The relative number of Ki-67-positive cells was calculated. The data are expressed as the mean ± SD. ***P < 0.001 versus NC. n = 3