Fig. 4
From: circ-TFRC downregulation suppresses ovarian cancer progression via miR-615-3p/IGF2 axis regulation
miR-615-3p and IGF2 were the downstream targets of circ-TFRC. (A) Bioinformatics analysis showed the downstream target of circ-TFRC using different websites. (B and C) Luciferase activity of circ-TFRC in HEK293T cells transfected with miR-615-3p mimics, which were putative binding sites for the circ-TFRC sequence. Luciferase activity was normalized by Renilla luciferase activity. n = 3. (D) Bioinformatics analysis showed the downstream target of miR-615-3p using different websites. (E and F) Relative luciferase activity determined 48 h after the transfection of HEK293T cells with miR-615-3p mimic/NC or IGF2-3’UTR WT/Mut. The data are expressed as mean ± SD. ***P < 0.001. n = 3