Fig. 5

The comparison of the immunosuppressive capacity upon T lymphocytes between HD-MSCs and MM-MSCs. A Representative FCM diagram of enriched total CD3⁺ T cells from PBMCs by MACS. B T cell counting after 3-day’s culture alone (Ctr) or coculture with HD-MSCs (T + HD) or MM-MSCs (T + MM). C, D Representative FCM diagram C and statistical analysis D of CD3⁺CD4⁺ T cells and CD3⁺CD8⁺cells after 3-day’s culture. E, G Representative FCM diagram E and statistical analysis F of Th1 (CD3⁺CD4⁺CD183⁺CD196⁻) and Th2 (CD3⁺CD4⁺CD183⁻CD196⁻), together with Th17 (CD3⁺CD4⁺CD183⁻CD196⁺) proportions G after 3-day’s culture. All data were shown as mean ± SEM (N = 3 independent experiments). NS not significant; *P < 0.05; **P < 0.01; ****P < 0.0001