Fig. 2

CCDC25 reduced the proliferation and migration of ccRCC in vitro. A CCDC25 protein levels in normal renal cell line (HK2) and ccRCC cell lines (Caki-1, 786–0, A498 and Caki-2) measured by western blotting. B Overexpression efficiencies of CCDC25 in Caki-1 and 786–0 cells was detected by Western blot analysis. C CCK-8 assays were used to detect the proliferation of Caki-1 and 786–0 cells after overexpression CCDC25 (TWO-WAY anova, * P < 0.05). D EdU assays and the relative quantitative results of cell proliferation are shown in histogram form (*p < 0.05, **p < 0.01). E Representative images and bar graphs of Transwell migration assays of Caki-1 and 786–0 cells after overexpression CCDC25 (**p < 0.01, ***p < 0.001,). F Apoptosis assays and the relative quantitative results. G Representative images and bar graphs of wound healing assays of Caki-1 and 786–0 cells after overexpression CCDC25 (**p < 0.01)