Fig. 3

CCDC25 suppress Hippo pathway by promoting YAP phosphorylation and inhibiting its nuclear entry. A Volcano plot showing the differential genes in the 786–0-CCDC25 cell cluster. B KEGG plot showing pathway enrichment of differentially overexpressed genes in 786–0-CCDC25 cell cluster. C, D Representative immunoblots of biomarkers of proliferation and the Hippo pathway, using ACTIN as reference. Quantification of protein expression revealed that overexpressed CCDC25 notably inhibited the expression of downstream Hippo pathway proteins ITGA3 and CCND1, and elevated the expression levels of p-YAP. E, F Representative immunoblots of biomarkers of EMT pathway, using ACTIN as reference. Quantification of protein expression revealed that overexpressed CCDC25 notably elevated the expression levels of E-cadherin and inhibited the expression of N-cadherin and Vimentin. G Nuclear levels of YAP protein, using Lamin B1 as reference. Quantification of protein expression revealed that overexpressed CCDC25 notably reduced nuclear levels of YAP protein. H Dual-luciferase activity assay on the ITGA3 promoter, YAP and CCDC25. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ns, no significance. Data are representative of three independent experiments in each group