Fig. 4

CCDC25 affects the Hippo pathway through YAP. A Overexpression efficiencies of YAP in Caki-1 and cells was detected by Western blot analysis. C Overexpression efficiencies of YAP in 786–0 and cells was detected by Western blot analysis. B, D Representative immunoblots of biomarkers of the Hippo pathway, using ACTIN as reference. Quantification of protein expression revealed that overexpression of YAP reversed the reduction of ITGA3 and CCND1 caused by CCDC25 overexpression. E 293 T cell lysates were prepared by weak RIPA lysis and co-immunoprecipitated by CCDC25 antibody with IgG as negative control. The result showed that YAP interacts with CCDC25. F Sub-localizations of YAP and CCDC25 were analyzed using immunofluorescence. Laser Confocal Microscopy photos showed that YAP and CCDC25 were co-localization both in the nuclei and cellular matrix. The scale bars represent 50 μm