Gene | Sequence of Primers | Annealing temperature |
---|---|---|
GAPDH | Forward: 5ʹ-TGCCGCCTGGAGAAACC-3' | 60 °C |
Reverse: 5ʹ-TGAAGTCGCAGGAGACAACC-3' | ||
U6 | Forward: 5ʹ-GCTTCGGCAGCACATATACTA-3' | 60 °C |
Reverse: 5ʹ-CGAATTTGCGTGTCATCCTTG-3' | ||
SNHG16 (NR_027821) | Forward: 5ʹ-TCCTCCTCCTTGGGTGCTCT-3' | 60 °C |
Reverse: 5ʹ-CCTTACATCCCTGCCTCCTCTA-3' | ||
KCNQ1OT1 (NR_001461.5) | Forward: 5ʹ-GCACTCTGGGTCCTGTTCTC-3' | 60 °C |
Reverse: 5ʹ-CACTTCCCTGCCTCCTACAC-3' | ||
miR-132-3p (MI0000158) | Forward: 5ʹ-CAGTCTACAGCCATGGTC-3' | 60 °C |
Reverse: 5ʹ-GAACATGTCTGCGTATCTC-3' | ||
PVT1 (NR_003368.2) | Forward: 5ʹ-ATCCACCCTCTTGTCTGATTTTCT-3' | 60 °C |
Reverse: 5ʹ-AATCCACAGGGTTCAGGAAGTC-3’ | 60 °C | |
Col1a2 (NM_007743) | Forward: 5ʹ-TTCTGTGGGTCCTGCTGGGAAA -3’ | 60 °C |
Reverse: 5ʹ-TTGTCACCTCGGATGCCTTGAG -3' | ||
Itih4 (NM_018746) | Forward: 5ʹ-CCTTTCCTGGAGAAGATGGCAC-3’ | 60 °C |
Reverse: 5ʹ-CTGATGAGAGCAGTGGATTGGC -3’ | ||
Mmp7 (NM_010810) | Forward: 5ʹ-AGGTGTGGAGTGCCAGATGTTG -3’ | 60 °C |
Reverse: 5ʹ-CAGTGAAATTCTTGACCGCTTTCC-3' | ||
Msx2 (NM_013601) | Forward: 5ʹ-AAGACGGAGCACCGTGGATACA -3’ | 60 °C |
Reverse: 5ʹ-CGGTTGGTCTTGTGTTTCCTCAG-3’ | ||
NEAT1 | Forward: 5ʹ-GCTCTGGGACCTTCGTGACTCT-3' | 60 °C |
Reverse: 5ʹ-CTGCCTTGGCTTGGAAATGTAA-3’ |