Figure 3

ECM microtopography modulates Rho A activity in less and more tumorigenic colon cancer cells. When plated on either a pit or a post microtopography, Caco-2 E cells exhibited a significant decrease in Rho A activity as compared to control (p < 0.01). In contrast, SW620 cells exhibited an increase in activity as compared to control when plated on either a pit or a post microtopography. In both Caco-2 E and SW620 cells, Rho A activity was higher on a post microtopography than on a pit microtopography. When plated on either a pit or a post microtopography, the Rho A activity of SW620 cells was significantly higher than that of Caco-2 E cells (* = p < 0.01). Rho A activity was determined using a colorimetric Rho A activity assay from Cytoskeleton, Inc.