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Figure 3 | Cancer Cell International

Figure 3

From: Morphologic transformation of human breast epithelial cells MCF-10A: dependence on an oxidative microenvironment and estrogen/epidermal growth factor receptors

Figure 3

Effect of PHR Presence in -HC/-EGF Medium on the Appearance of MCF-10A Transformed Foci After 3 Weeks of Continuous Culture. MCF-10A cells were seeded in triplicate at a density of 6.25 × 104 cells/well in 6-well tissue culture plates and grown in -HC/-EGF medium either with or without phenol red for 3 weeks in continuous culture. Cells were stained with neutral red (10 mg/ml) and photographed (10× obj.). Photographs are representatives of triplicate wells per culture condition from one experiment.

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