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Table 2 Reversibility of Morphologic Transformation due to Re-addition of PHR, HC, and/or EGF to MCF-10A Cells Grown for 5 Weeks in -PHR/-HC/-EGF Medium

From: Morphologic transformation of human breast epithelial cells MCF-10A: dependence on an oxidative microenvironment and estrogen/epidermal growth factor receptors

Group Culture Condition Mean Number of Foci/Well +/- SE
1 -PHR/-HC/-EGF 153.50 +/- 0.50
  Add Back PHR 157.50 +/- 4.50
2 -PHR/-HC/-EGF 125.67 +/- 4.98
  Add Back HC 75.33 +/- 6.74*
3 -PHR/-HC/-EGF 79.50 +/- 6.50
  Add Back EGF 92.50 +/- 12.50
4 -PHR/-HC/-EGF 145.50 +/- 12.00
  Add Back HC & EGF 156.50 +/- 12.50
  1. MCF-10A cells, plated at 5 × 105 cells/well, were grown in -PHR/-HC/-EGF medium for 5 weeks in 6-well tissue culture plates, at which time cells were re-exposed to depleted agents once for 1 week. Foci were counted at weeks 5 and 6 and the average number of foci/well in 2 wells per re-added agent(s) before and after re-supplementation from one experiment was used to assess reversibility of morphologic transformation. Significance was analyzed using One-way ANOVA followed by Tukey's test.
  2. Add Back HC vs -PHR/-HC/-EGF *p < 0.01