Morphologic transformation of human breast epithelial cells MCF-10A: dependence on an oxidative microenvironment and estrogen/epidermal growth factor receptors

Table 2 Reversibility of Morphologic Transformation due to Re-addition of PHR, HC, and/or EGF to MCF-10A Cells Grown for 5 Weeks in -PHR/-HC/-EGF Medium

MCF-10A cells, plated at 5 × 10⁵ cells/well, were grown in -PHR/-HC/-EGF medium for 5 weeks in 6-well tissue culture plates, at which time cells were re-exposed to depleted agents once for 1 week. Foci were counted at weeks 5 and 6 and the average number of foci/well in 2 wells per re-added agent(s) before and after re-supplementation from one experiment was used to assess reversibility of morphologic transformation. Significance was analyzed using One-way ANOVA followed by Tukey's test.
Add Back HC vs -PHR/-HC/-EGF *p < 0.01

ISSN: 1475-2867