Knockdown of EFNA1 and EPHA2 suppresses growth in semi-solid medium. A) HeLa cells were transfected with pcDNA3 empty vector control, EFNA1 shRNA1, EFNA1 shRNA2. Transfected cells were plated in 0.5% agarose and allowed to grow for two weeks. Shown are ten representative fields for each transfection. B) Quantification of growth in semi-solid medium. Results represent the average number of colonies +/- standard deviation from three independent experiments each plated in quadruplicate. C) Western blot of knockdown of endogenous EPHA2 showing typical knockdown of using siRNA directed against EPHA2 (Top). Below is the same blot reprobed with anti-β-actin, which was used to confirm equal loading between lanes. D) Quantification of growth in semi-solid medium as above. In this case, HeLa cells were transfected with siRNAs against EFNA1 or EPHA2.