Figure 4

Morphology of differentiated osteoblast cells. (A) Morphology of differentiated murine mononucleated cells. (B) Morphology of differentiated MC3T3-E1 cells. Both cells were cultured in osteoblast differentiation media for 5, 7, 10 or 14 days prior to morphological analysis by von Kossa staining. The black arrows show the formation of light-brown, dark-brown or black nodules (400× magnification).