Table 1 Primer sequences used in Reverse-transcription polymerase chain reaction (RT-PCR)
From: Chalcone-imidazolone conjugates induce apoptosis through DNA damage pathway by affecting telomeres
S.NO | Primer | Primer Sequence | Primer length | Tm(0C) |
|---|---|---|---|---|
1. | hTERT, Size: 214bp | |||
| Â | Sense primer | 5'-cgtggtttctgtgtggtgtc-3' | 20 | 60 |
| Â | Anti sense primer | 5'-ccttgtcgcctgaggagtag-3' | 20 | 60 |
2. | TRF2, Size: 160bp | |||
| Â | Sense primer | 5'-gtacccaaaggcaagtggaa-3' | 20 | 59.97 |
| Â | Anti sense primer | 5'-tgacccactcgctttcttct-3' | 20 | 59.99 |
3. | TRF1, Size:212bp | |||
| Â | Sense primer | 5'-tctctctttgccgagctttc-3' | 20 | 59.84 |
| Â | Anti sense primer | 5'-ggctgattccaagggtgtaa-3' | 20 | 59.93 |
4. | TIN2, size: 208bp | |||
| Â | Sense primer | 5'-ctgagcccatggaacagaat-3' | 20 | 60.67 |
| Â | Anti sense primer | 5'-tccttatggcctcccctagt-3' | 20 | 59.92 |
5. | P16, size: 171bp | |||
| Â | Sense primer | 5'-gggtcgggtagaggaggtg-3' | 19 | 61.85 |
| Â | Anti sense primer | 5'-gcgctacctgattccaattc-3' | 20 | 59.67 |