Figure 4

Soluble MICA and MICB in supernatants from human cervical cancer cell lines and non-tumorigenic keratinocytes. Levels of soluble MICA and soluble MICB were measured in concentrated supernatants of HaCaT, C33-A, SiHa and HeLa cells after three days of cell culture by ELISA assays using the corresponding anti-MICA or anti-MICB monoclonal antibodies. Absorbance values (at A₄₅₀) measured by duplicate were plotted against standard dilutions and were expressed as pg/mL.