Relative quantification analysis of MICA and MICB genes. A) Relative expression of MICA and MICB in each cell line was performed by Real-Time PCR using GAPDH, β-Actin and L32 ribosomal protein as reference genes. The "fold expression" (ordinates) was obtained by normalizing the data values taking MICA expression of HaCaT cells as 1. The figure represents the average ± SD of the relative expression obtained with the reference genes. B) Temperature melting analysis performed by Real-time RT-PCR shows amplification of only one specific peak for each set of primers. C) Specificity of MICA and MICB amplification fragments was demonstrated by running the amplification products on 1.5% agarose gel, giving a fragment of approximately 448 bp for MICA and 350 bp for MICB.