Figure 2

Apoptotic index. (A) The panels of flow cytometric analysis of apoptotic index. According to kits' instruction manual, quadrants of panels were defined as: the lower left quadrant of density plots for the numbers of viable cells (annexin V-; PI-), the lower right quadrant for the numbers of earlier stages of apoptotic cells (annexin V+; PI-), the upper right quadrant for the numbers of late stages of apoptotic cells (annexin V+; PI+), and the upper left quadrant for the numbers of dead cells. In the graph only the percentage of apoptotic cells (lower left and upper left) were presented. (B) The graph of apoptotic index. The columns in the graph represent the sum of earlier and late stages of apoptotic cells.(C) The graph of caspase-3 levels. Nos, noscapine; IM, imatinib mesylate; Nos+IM, noscapine with imatinib mesylate. Results are the mean of three different experiments and presented as mean ± SEM.