Figure 3

Vitamin K3 (VK3) alone induces simultaneous activation of the transcription factor NF-κB, p53, c-Jun and protease caspase-3 in Jurkat cells. Jurkat cells were left untreated (A, C, E, G) or exposed to (10 μM) VK3 (B, D, F, H), at 37 °C for 24 h. After this time of incubation, cells were stained with anti-NF-κB-p65 (A, B), anti-p53 (C, D), anti-c-Jun (E, F) and anti-caspase-3 (G, H) antibodies according to procedure described in Materials and Methods. Notice that NF-κB, p53, c-Jun and caspase-3 (CASP-3) positive-nuclei (dark brown color) reflect their nuclear translocation/activation and appear to correlate with the apoptotic nuclear morphology, i.e. condensed/fragmented nuclei compared with untreated cells (A, C, E, G) or cytoplasmic activation (brown color). Magnification 660x (A-H). Inset Magnification (B, 2000x; D:1100x).