Table 3 Summary of primer pairs for PCR
Name | Annealing temperature | Mean product size | Primer | Sequence (5' to 3') |
|---|---|---|---|---|
Ccr7 | 61°C | 208 bp | forward | AGCACCATGGACCCAGGGA |
| Â | Â | Â | reverse | CTGCCTCTCATGTATTCTGT |
Cxcr4 | 57°C | 390 bp | forward | GGCTGTAGAGCGAGTATTGC |
| Â | Â | Â | reverse | GTAGAGGTTGGTGACAGTGTAGAT |
Cxcl12 | 61°C | 512 bp | forward | ACACTCCGCCATAGCATATGGT |
| Â | Â | Â | reverse | TGAAGCATGCGTTTGGAGG |
EGFP | 61.5°C | 220 bp | forward | GACAAGCAGAAGAACGGCATCAAG |
| Â | Â | Â | reverse | CGGCGGCGGTCACGAACT |
HYG | 60.5°C | 500 bp | forward | AGCTGCGCCGATGGTTTCTACAA |
| Â | Â | Â | reverse | ATCGCCTCGCTCCAGTCAATG |
Syn A | 60°C | 281 bp | forward | TACCTGATGCGCCTGGAGCT |
| Â | Â | Â | reverse | AAGCTTTGCAGGAACTGGAGAA |
Syn B | 60°C | 201 bp | forward | CCACCACCCATACGTTCAAA |
| Â | Â | Â | reverse | GGTTATAGCAGGTGCCGAAG |
Slc1a5 | 59°C | 585 bp | forward | CTGGATTATGTGGTACGCCAC |
| Â | Â | Â | reverse | GACCTGTCCACTAGCCAGTC |
Dhfr | 59°C | 159 bp | forward | CCACAACCTCTTCAGTGGAAGGTAAACAGA |
| Â | Â | Â | reverse | TTGGCAAGAAAATGAGCTCCTCGTGG |
Gapdh | 68°C | 980 bp | forward | TGAAGGTCGGTGTGAACGGATTTGGC |
| Â | Â | Â | reverse | CATGTAGGCCATGAGGTCCACCAC |