Effect of chlorpromazine or amiloride on TM601 uptake by NHDF fibroblasts. Western blots of TM601 (10 μM) - treated NHDF fibroblasts in presence or absence of 300 μM amiloride or 10 μg/ml chlorpromazine (A). Lysates of trypsinized cells were used at 9-12 μg protein. Beta-actin was used as a sample loading control (representative data of n = 3). The statistically significant 2 fold (p < 0.05) effect of amiloride (Amilor) on TM601 (TM) level is shown on the graph (B). The chlorpromazine had a small, statistically not significant effect on TM601 level (A). The data represent n = 3 experiments using duplicate samples/per experiment (total of 6 samples).