Skip to main content
Figure 5 | Cancer Cell International

Figure 5

From: Upstream molecular signaling pathways of p27 (Kip1) expression in human breast cancer cells in vitro: differential effects of 4-hydroxytamoxifen and deficiency of either D-(+)-glucose or L-leucine

Figure 5

Effects of tamoxifen, 4-hydroxytamoxifen, and deficiency of D -(+)-glucose, L -leucine, L -methionine or L -cysteine on the phosphorylation of S6K1 in MDA-MB-231 cells. (a and e) Western immunoblot analysis of the effects of D-(+)-glucose deficiency, DMSO, tamoxifen and 4-hydroxytamoxifen on the expression of total S6K1 and (b and e) phosphorylated S6K1. (c and e) Westerm immunoblot analysis of the effects of the deficiency of L-leucine, L-methionine or L-cysteine on the expression of total S6K1 and (d and e) phosphorylated S6k1. All assays were performed in triplicates and repeated three times. (f) Correlation between the degree of expression of p27 protein and the degree of down-regulation of phosphorylated S6k1.

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us